fig2

Figure 2. Ultrastructural analysis of intranuclear inclusions (NI) in hepatocellular carcinoma. A: The image depicts two nuclei, one of which contains a large NI, which we analyzed in more detail at higher magnification; B: NI is bordered by the two (inner and outer) nuclear membranes (arrowhead) with attached heterochromatin (h); degraded cellular material resembling fine filamentous material (asterisk), a small vesicle with homogenous content of high electron-density, most probably a lysosome (black arrow) and heterolysosomes (white arrows) are seen. In addition, the NI shows fine granular, ribosome-like material (triangles) and a larger round area with no clearly detectable membrane (#) and hardly electron-dense content, which might be a lipid droplet