fig1

Figure 1. Intranuclear inclusions in hepatocellular carcinoma. Positive immunoreactivity for β-catenin, glutamine synthethase and KDM2A in intranuclear inclusions (NI). The images depict NI (arrows) containing accumulations of β-catenin, glutamine synthethase and KDM2A. For β-catenin and glutamine synthethase IHC increased magnification of the marked area shows positive immunostainings within NI. Further, HE-staining (bottom-left image) reveals that NI (arrows) are completely closed with no contact to the cytoplasm on this plane. Additionally increased magnification of the intranuclear inclusion in the center of the HE and KDM2A-images reveal the bordering membrane (right images; arrow). The black bars at the right (third and forth row) equal 10 µm. First and second row images are original magnifications: 400 ×; third and fourth row images are original magnifications: 1,000 ×