fig3

Figure 3. (a-d) RAPD profi les of genomic DNA from cell line of hepatoma (H4IIE-luc) of rats following exposure to FB₁ and/or AFB₁ for various time periods. Four pictures represents PCR products with primer OPD07 (a), OPD09 (b), OPD15 (c), and OPD16 (d), respectively, at less and greater concentration. The DNA marker (100 bp) in lanes 1 and 2 represents cells only; lane 3 represents cells plus FB₁ (0.1 μmol/L); lane 4 represents cells plus FB₁ (200 μmol/L); lane 5 represents cells plus AFB₁ (0.025 μmol/L); lane 6 represents cells plus AFB₁ (50 μmol/L); lane 7 represents cells plus the mixture of FB₁ and AFB₁ (0.1 + 0.025 μmol/L); lane 8 represents cells plus the mixture of FB₁ and AFB₁ (200 + 50 μmol/L); lane 9 represents cells plus Amaranthus hybridus extract (40 μg/mL); lane 10 represents A. hybridus extract(40 μg/mL) plus FB₁ (0.1 μmol/L); lane 11 represents Amaranthus hybridus extract (40 μg/mL) plus FB₁ (200 μmol/L); lane 12 represents Amaranthus hybridus extract (40 μg/mL) plus AFB₁ (0.025 μmol/L); lane 13 represents Amaranthus hybridus extract (40 μg/mL) plus AFB₁ (50 μmol/L); lane 14 represents Amaranthus hybridus extract (40 μg/mL) plus the mixture of FB₁ and AFB₁ (0.1 + 0.025 μmol/L), and lane 15 represents Amaranthus hybridus extract (40 μg/mL) plus the mixture of FB₁ and AFB₁ (200 + 50 μmol/L). FB₁: fumonisin B₁; AFB₁: aflatoxin B₁; RAPD: random amplification of polymorphic DNA; PCR: polymerase chain reaction